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. 2011 Feb 28;6(2):e17321. doi: 10.1371/journal.pone.0017321

Figure 2. hNGF mutant bioactivity on survival and differentiation of PC12 cells, neuroblastoma SH-SY5Y cells and chick DRG neurons.

Figure 2

In Panels A–C, PC12 cells were plated in presence of 100 ng/ml of hNGF (B) or hNGFR100E (C) and the number of PC12 processes evaluated (J). In panels D-F, PC12cells were primed with 50 ng/ml of hNGF (E) or hNGFR100E (F) for 1 week and replated for 2 days in presence of 10 ng/ml of either hNGF or hNGFR100E. Negative controls (A,D) are represented by cells incubated in absence of hNGF or hNGFR100E. (G) Untreated human neuroblastoma SH-SY5Y cells are induced to differentiate when treated for 7 days with 100 ng/ml of hNGF (H), or with hNGFR100E (I). The mutant hNGFR100E is as effective as wild type hNGF in determining the survival and differentiation of chick embryo dorsal root ganglia sensory neurons, after a 48 hrs exposure.