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. 2011 Feb 28;6(2):e17364. doi: 10.1371/journal.pone.0017364

Figure 6. Inhibition of IL-17 production by selective cytokine neutralization.

Figure 6

A) Inhibition of IL-17 production by selected couples or cocktail of cytokine blocking antibodies. Naïve CD4+ T cells were activated with anti-CD3 coated beads and autologous DC at a cell ratio of 1∶4 in the presence of the indicated couples of cytokine-neutralizing antibodies, or of the respective control isotypes. The presence of IL-17 was analyzed in the supernatants of cell cultures after 6 days by ELISA. The effect of the control isotypes couples on IL-17 production was not significantly different from that of the control isotypes cocktail. The values represent the mean +/− SD of three independent experiments. Statistical analysis was performed by Mann-Whitney test. * p<0.05. B) Inhibition of IFN-γ and IL-23 production by selective IL-23/IL-12 neutralization as compared to the inhibition obtained with the complete neutralizing cocktail, including antibodies to the p40 subunit of IL-12 and IL-23, p19 (IL-23), IL-1β, TNF-α and IL-6. Th values represent the mean +/− SD of three independent experiments. Statistical analysis was performed by Mann-Whitney test. * p<0.05.