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. 2011 Feb 28;6(2):e17424. doi: 10.1371/journal.pone.0017424

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Yin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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RNAs were isolated from EHEC O157:H7 strain 86-24 grown in BHIN, BHIN+bs, BHIN+lac, BHIN+bs+lac and MB at 37°C overnight statically, and the bacteria recovered from loops inoculated with bacteria grown in BHIN (BHIN-Loop) and MB (MB-Loop) at 37°C overnight statically as well as the control loops inoculated with EMEM (Control-Loop). Data are expressed as the means ± SD for RNA extracted in 4–6 biological replicates. Relative fold expression (RFE) represents the changes in transcription compared to the bacteria grown in BHIN (value of 1.0). The levels of 16SrRNA transcripts were used to normalize the C_t values. * indicates p<0.05 as compared to BHIN; † indicates p<0.05 as compared to BHIN-Loop.