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. 2011 Feb 28;6(2):e17424. doi: 10.1371/journal.pone.0017424

Figure 6. Transcriptional profiles of genes for putative adhesins, toxins, protease and secreted proteins.

Figure 6

RNAs were isolated from EHEC O157:H7 strain 86-24 grown in BHIN, BHIN+bs, BHIN+lac, BHIN+bs+lac and MB at 37°C overnight statically and the bacteria recovered from loops inoculated with bacteria grown in BHIN (BHIN-Loop) and MB (MB-Loop) at 37°C overnight statically as well as the control loops inoculated with EMEM (Control-Loop). Data are expressed as the means ± SD for RNA extracted in 4-6 biological replicates. Relative fold expression (RFE) represents the changes in transcription compared to the bacteria grown in BHIN (value of 1.0). The levels of 16SrRNA transcripts were used to normalize the Ct values. * indicates p<0.05 as compared to BHIN; † indicates p<0.05 as compared to BHIN-Loop.