Figure 2. Effect of L.s.-P and its fractions L.s.-1.0 and L.s.-1.25 on PMN adhesion to platelet-coated surface under flow conditions.

Polysaccharide fractions L.s.-1.0 and L.s.-1.25 or a parent mixture L.s.-P were added at a final concentration of 100 µg/ml to a platelet-coated surface and incubated for 10 min at RT. The same concentration of each compound was also added to non-treated (filled bars) or IB4-pretreated (grey bars) PMN suspensions before addition to platelets. Under the flow conditions, migration of PMN was monitored and photographed. Analysis was performed by counting the number of attached PMN per field in at least 4 different fields. The mean percentage ± SEM with respect to control of at least three independent experiments is represented. *P<0.05; **P<0.01.