Figure 4. (A-B) Specific effect of L.s.-P and its fractions L.s.-1.0 and L.s.-1.25 on the inhibition of bFGF-induced HUVEC tubulogenesis.

(A) Representative photographs of HUVEC cultured on Matrigel in the presence of bFGF along with 100 µg/ml of the indicated polysaccharide preparations. (B) Quantitative analysis of tube-like structures. All data were expressed as the percentage of tubes/cm² vs control (bFGF). (C,D) Specific effect of L.s.-P and its fractions L.s.-1.0 and L.s.-1.25 on the inhibition of Balb/c 3T3 adhesion to bFGF. (C) Effects of L.s.-P and L.s.-1.25 on fibroblast cell adhesion to bFGF. Representative images of fibroblast cell adhesion to purified bFGF are shown. The images are representative of three independent experiments. Quantification was performed by counting adhered cells of at least three different fields. Results are expressed as percentage of the treated sample with respect to control (D). ***P<0.001.