Table 1. Sulfate content, anti-inflammatory and anti-coagulant activities of the polysaccharide preparations from L. saccharina brown seaweed.

Sample	Inflammation b			APTT, U/mg c	Sulfate content
	Dose mg/kg	Number of PMNs per rat (×106)	Inhibition (% to control)
Controla	-	49.1±5.5	-	-	-
L.s.-P	1.04.0	7.6±2.73.2±0.6	84.593.4	36.4±1.7	28.8
L.s.-1.0	1.04.0	16.2±1.58.4±1.3	67.082.9	6.8±0.2	16.2
L.s.-1.25	1.04.0	5.8±1.92.2±0.6	88.295.5	29.2±1.6	36.8

^aSix animals received 0.9% (wt/vol) NaCl instead of polysaccharides.

^bThe anti-inflammatory activity was determined as the effect on PMN transmigration to the peritoneal cavity of rats (six animals in each group, for details see Results ). The polysaccharide preparations were injected intravenously with a dose of 1.0 and 4.0 mg/kg of the rat weight. Data are presented as mean ± SEM. N is the number of rats in the group.

^cAnticoagulant activity was measured as the APTT related to a heparin standard with an activity of 140 U/mg. Data are shown as mean ± SEM; N = 4.