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. 2011 Feb 28;6(2):e17414. doi: 10.1371/journal.pone.0017414

Figure 3. NLRs that activate caspase-1 do not signal in response to B. burgdorferi ligands.

Figure 3

BMDM were stimulated with B. burgdorferi (MOI 10) for 6 hours. In designated samples, 5 mM ATP was added for the last 2 hours of incubation to activate the caspase-1 inflammasome and induce IL-1β secretion. Concanamycin A (100 ng/ml) was added 30 minutes prior to stimulation. Cytokine levels in supernatants were measured by ELISA. Shown is 1 representative experiment from 3 independent experiments. Bars represent the mean secretion of IL-1β in pg/ml ± SD of triplicates in the experiment, *p<0.05.