Skip to main content
. 2011 Feb 28;6(2):e17027. doi: 10.1371/journal.pone.0017027

Figure 3. A. baumannii OMVs deliver virulence factor AbOmpA into host cells.

Figure 3

(A) HeLa and HEp-2 cells were treated with A. baumannii OMVs (5 µg/ml of protein concentrations) for 12 h. The cells were fixed, permeabilized, and stained with anti-rabbit AbOmpA antibody, followed by Alexa Fluor® 568-conjugated rabbit IgG (red). DAPI was used to stain the nuclei (blue). Magnification: ×400. The U937 cells were treated with 5 µg/ml of A. baumannii OMVs for 4 h and then stained with anti-rabbit AbOmpA antibody, followed by Alexa Fluor® 488-conjugated rabbit IgG (green). Magnification: ×1,260. (B) Western blot analysis of cell lysates. The differentiated U937 cells were treated with A. baumannii OMVs (20 µg/ml of protein concentrations) for the indicated times. Cell lysates were separated on 12% SDS-PAGE, transferred to membranes, and immunoblotted with a rabbit anti-AbOmpA immune serum and β-actin antibody.