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. 2011 Feb 28;6(2):e17027. doi: 10.1371/journal.pone.0017027

Figure 4. A. baumannii OMVs interact with plasma membrane of host cells through a cholesterol-rich membrane microdomain.

Figure 4

(A) HeLa cells were treated with OMVs (5 µg/ml of protein concentrations) for 4 h. (B) HeLa cells were preterated with 10 mM MβCD for 45 min and then treated with OMVs for 4 h. The cells were fixed, permeabilized, and stained with anti-rabbit AbOmpA antibody, followed by Alexa Fluor® 488-conjugated rabbit IgG (green). DAPI was used to stain the nuclei (blue). Magnification: ×630.