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. 2011 Feb 28;6(2):e17027. doi: 10.1371/journal.pone.0017027

Figure 6. Host cell entry and cytotoxicity of rAbOmpA fragments.

Figure 6

(A) HeLa cells were treated with different forms of micelles containing rAbOmpA fragments for 24 h. The cells were fixed, permeabilized, and stained with anti-rabbit AbOmpA antibody, followed by Alexa Fluor® 568-conjugated rabbit IgG (red). DAPI was used to stain the nuclei (blue). The subcellular localization of AbOmpA was observed by confocal microscopy. Magnification: ×400. (B) The differentiated U937 cells were treated with different forms of micelles containing rAbOmpA fragments for 24 h. Cell viability was determined by a WST-1 assay. Untreated control cells (⧫), rAbOmpA22-170 (•), rAbOmpA1-356 (X), and rAbOmpA221-339 (▪).