Figure 7. Displacing effect of HSA site specific markers on the ICD signal of creatinine-HSA complex.

Results of CD displacement experiments performed with warfarin, phenylbutazone, ibuprofen and diazepam on HSA-CTN complex at (A) pH 7.0 and (B) pH 9.0 (l = 1 cm, T = 25°C). Displacers were added as µl aliquots of stock solutions. Positive induced CD values measured at 278 nm are plotted against displacer/creatinine molar ratios (for further details see Materials and methods).