Table 2. Association constants and thermodynamic data for binding of creatinine to HSA.

Condition	Ka[M−1]		n		ΔGa[Kcal/mol]	ΔHa[Kcal/mol]	ΔSa[cal/mol/K]
	ITCa	Specb	ITCa	Specb
A	8.92×104	*1.15×104 #2.92×104	0.98	*0.94#1.07	−6.75	−0.017±0.91	22.6
B	2.69×105	*9.76×105 #1.36×105	0.94	*1.29#1.17	−23.03	−15.53±0.79	−25.2

Condition A: N Isomer of HSA at pH 7.0+ CTN.

Condition B: B Isomer of HSA at pH 9.0+ CTN.

^aConstants determined by Isothermal Titration Calorimetry.

^bConstants determined by Fluorescence spectroscopy (^*λ_excitation = 280 nm; ^#λ_excitation = 295 nm).