Fig. 3.
1H-15N HSQC spectra of uniformly 15N labeled HCV p7. (A) p7 in micelles in H2O. (B) p7 in micelles in D2O. (C) p7 in micelles with Mn2+-EDTA. (D) p7 in isotropic bicelles. The micelle samples contained 125 mM DHPC. For deuterium exchange experiments the sample was prepared in 75% D2O. Mn2+-EDTA at a concentration of 10 mM was added to the sample for PRE experiments. The isotropic bicelle sample contained DMPC and DHPC (q = 0.5).