Skip to main content
. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2010 Dec 2;79(4):1179–1187. doi: 10.1016/j.ijrobp.2010.10.003

Fig. 1.

Fig. 1

Sensitivity of head and neck squamous cell carcinoma (HNSCC) cell lines HN5 and FaDu to treatment with A12 in combination with radiation. (A) Western blot analysis of IGF-1R expression. (B) The cells were incubated with increasing concentrations of A12 (1–100 nM) and viability of treated cells relative to untreated cells at 72 hours was assessed using MTT colorimetric assay. Statistical significance of A12 effect was reached for HN5 at 10 nM and for FaDu at 100 nM dose levels (p<0.05). (C) Effect of A12 on radiosensitivity of HNSCC cells in culture. Cells were plated in defined cell numbers for 24 hours, then treated with A12 (100 nM) for five hours before radiation. The numbers of colonies were counted 14 days later and the survival curves were constructed with normalized values for the cytotoxicity induced by A12 alone. The average results of three independent experiments (HN5, *p<0.05, EF=1.74 at 0.5 survival fraction) or representative single experiments in triplicate (FaDu) are shown. Bars represent ± SE.