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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2010 Dec 2;79(4):1179–1187. doi: 10.1016/j.ijrobp.2010.10.003

Fig. 2.

Fig. 2

Effects of IGF1 on cell viability and radiation response of FaDu tumor cells in vitro. The cells were plated in 10% serum and allowed to attach overnight, then they were incubated in serum deprived (0.5%) medium for 72 hours. At 24 hours after start of serum deprivation the cells were irradiated. A12 was added four hours before IGF1 and five hours before radiation. (A) Immuno-precipitation and Western blot analysis of FaDu lysates after exposure to IGF-1, A12 or both. IGF-1R antibody was used to immuno-precipitate IGF-1R protein and p-IGF-1R antibody was used in immuno-blot analysis. (B) Cell viability was assessed at 48 hours after radiation (and 72 hours after serum deprivation) using the MTT colorimetric assay. *p<0.05. (C) Radiation cell survival curves with or without A12. A12 significantly enhanced radiation response of FaDu to 5 Gy (p<0.05, EF=1.40 at 0.5 survival fraction). In A and B the results of two independent experiments (in triplicate) are shown. Bars represent ± SE.