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. Author manuscript; available in PMC: 2012 Mar 1.

Published in final edited form as: Biochim Biophys Acta. 2011 Jan 12;1813(3):438–447. doi: 10.1016/j.bbamcr.2011.01.003

Fig. 3 — (A) Mock-shRNA (Ctl) or DDX3 knockdown (KD) MCF-7 cells were treated with HDAC inhibitors 5 mM NaBu, or 1 μM TSA for 4 hr, and then the levels of Snail, DDX3 and β-actin were measured. (B) Snail and 18S mRNA levels were measured in mock-shRNA (Ctl) or DDX3 knockdown MCF-7 cells after treatment with 5 mM NaBu or 1 μM TSA for 3 hr. (C) Real time PCR was used to measure Snail and 18S mRNA levels in MCF-7 mock-shRNA and DDX3 knockdown cells after treatment with 5 mM NaBu or 1 μM TSA for 3 hr. Quantitative values are from NaBu or TSA treatment of DDX3 knockdown cells compared with mock-shRNA MCF-7 cells Means ± SEM, n=3, *p < 0.05. (D) Acetyl-histone H3 and total histone H3 levels were immunoblotted after mock-shRNA or DDX3 knockdown MCF-7 cells were treated with 5 mM NaBu for 4, 5, or 6 hr. (E) Mock-shRNA and DDX3 knockdown MCF-7 cells were treated with lithium (20 mM) for 30 min, 5.5 hr, with lithium for 30 min followed by the addition of 10 μM MG132 during the last 5 hr, or MG132 for 5 hr, and DDX3, Snail, phospho-serine9-GSK3β, and β-actin levels were measured. (F) Mock-shRNA and DDX3 knockdown MCF-7 cells as described in Figure 3E were pretreated with lithium (20 mM) for 30 min, followed by treatment with 5 mM NaBu or 1 μM TSA for 4 hr, and Snail and β-actin levels were measured.

Fig. 3 — (A) Mock-shRNA (Ctl) or DDX3 knockdown (KD) MCF-7 cells were treated with HDAC inhibitors 5 mM NaBu, or 1 μM TSA for 4 hr, and then the levels of Snail, DDX3 and β-actin were measured. (B) Snail and 18S mRNA levels were measured in mock-shRNA (Ctl) or DDX3 knockdown MCF-7 cells after treatment with 5 mM NaBu or 1 μM TSA for 3 hr. (C) Real time PCR was used to measure Snail and 18S mRNA levels in MCF-7 mock-shRNA and DDX3 knockdown cells after treatment with 5 mM NaBu or 1 μM TSA for 3 hr. Quantitative values are from NaBu or TSA treatment of DDX3 knockdown cells compared with mock-shRNA MCF-7 cells Means ± SEM, n=3, *p < 0.05. (D) Acetyl-histone H3 and total histone H3 levels were immunoblotted after mock-shRNA or DDX3 knockdown MCF-7 cells were treated with 5 mM NaBu for 4, 5, or 6 hr. (E) Mock-shRNA and DDX3 knockdown MCF-7 cells were treated with lithium (20 mM) for 30 min, 5.5 hr, with lithium for 30 min followed by the addition of 10 μM MG132 during the last 5 hr, or MG132 for 5 hr, and DDX3, Snail, phospho-serine9-GSK3β, and β-actin levels were measured. (F) Mock-shRNA and DDX3 knockdown MCF-7 cells as described in Figure 3E were pretreated with lithium (20 mM) for 30 min, followed by treatment with 5 mM NaBu or 1 μM TSA for 4 hr, and Snail and β-actin levels were measured.