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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Jan 12;1813(3):438–447. doi: 10.1016/j.bbamcr.2011.01.003

Fig. 4. Topoisomerase inhibitors increase Snail expression.

Fig. 4

(A) MCF-7 cells were treated with 100 μM etoposide for 5-24 hr, and the levels of Snail, p53, and β-actin were immunoblotted. (B) MCF-7, (C) SH-SY5Y, or (D) MDA-MB-231 cells, which express mutant p53, were treated with 10 μM camptothecin for 2, 3, or 4 hr following incubation in serum-free media for 2 hr, and then Snail, p53, and β-actin levels were immunoblotted. (E) Cytosolic and nuclear p53 and Snail levels were measured after MCF-7 cells were treated with 10 μM camptothecin for 2, 3, or 4 hr. CREB was immunoblotted as a nuclear marker and α-tubulin as a cytosolic marker.