Table 1. Oligonucleotides used for cloning.
| Construct a | Sequence (5′ to 3′) b | RE |
| −10.8/−8.3 | F - ATATCTCGAGCCACCCCAAACTCAGTGG | XhoI |
| R - ATTTGGATCCGGGAGAACTGGTCATGGC | BamHI | |
| −10.8/−6 | F - ATATCTCGAGCCACCCCAAACTCAGTGG | XhoI |
| R - TAAAGGTACCCAAGATGGCTGCTGCAGC | KpnI | |
| −6/−0.9 | F - ATATCTCGAGGCTGCAGCAGCCATCTTG | XhoI |
| R - ATTTGGATCCCTTCGGCCTGACTGACAG | BamHI | |
| −6/−3.7 | F - ATATCTCGAGGCTGCAGCAGCCATCTTG | XhoI |
| R - TAAAGGATCCGAGACTACAGGTGCGTGC | BamHI | |
| −3.7/ATG | F - ATATCTCGAGGCACGCACCTGTAGTCTC | XhoI |
| R - TAAAGGATCCGAACACGTCCATCCTGGC | BamHI | |
| −3.7/−0.9 | F - ATATCTCGAGGCACGCACCTGTAGTCTC | XhoI |
| R - ATTTGGATCCCTTCGGCCTGACTGACAG | BamHI | |
| −1.1/−0.6 | F - ATATCTCGAGCAGCTGAGATTCGAGCCC | XhoI |
| R - ATTTGGATCCGCCGCCTCACCTGGATGC | BamHI | |
| −0.9/ATG | F - ATATCTCGAGCTGTCAGTCAGGCCGAAG | XhoI |
| R - TAAAGGATCCGAACACGTCCATCCTGGC | BamHI | |
| −0.6/ATG | F - ATATCTCGAGCTGTCAGTCAGGCCGAAG | XhoI |
| R - TAAAGGATCCGAACACGTCCATCCTGGC | BamHI | |
| β-Syn | F - GACGAGTAAGCTTGACGTGTTCATGAAGGG | HindIII |
| R - AAATATGGATCCTTACTACGCCTCTGGCTCATAC | BamHI | |
| γ-Syn | F - GACGAGTAAGCTTGATGTCTTCAAGAAGGG | HindIII |
| R - AAATATGGATCCTTACTAGTCTCCCCCACTCTG | BamHI |
a pEGFP-PrP (prion protein) was a gift from Dr Catia Sorgato and was subcloned into pcDNA3.1+ using HindIII and EcoR1 restriction sites and is not listed. pcDNA3.1+α-Syn, pcDNA 3.1+NRF2, pcDNA3.1+MTF-1 have been described previously [50], [51] and are not listed.
b Restriction endonuclease recognition sites are bolded and underlined; each oligonucleotide has a 4–7 bp flanking tag.
RE = Restriction Endonuclease.