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. Author manuscript; available in PMC: 2012 Mar 7.
Published in final edited form as: DNA Repair (Amst). 2010 Dec 10;10(3):260–270. doi: 10.1016/j.dnarep.2010.11.007

Figure 1. AZT halts replication and induces SOS.

Figure 1

(A) AB1157 cultures were grown to early log phase and then treated with 100 ng/ml AZT or thymidine for the times indicated. At each time point, 2 ml of culture was removed, EdU was added to 40 μg /ml and cultures were incubated for an additional 5 minutes. The cells were then fixed with 90% methanol, labeled with AlexaFluor488 and analyzed as described in the materials and methods. Median fluorescence for 50,000 cells is shown at each time point (⦿ No Edu, △ Thymidine, ▲AZT). (B) Micrograph showing AB1157 cultures labeled with EdU and visualized with Alexa Fluor 488 at 0 and 30 minutes after the addition of 100 ng/ml AZT. (C) AB1157 cultures harboring a plasmid with the luxCDABE operon under control of the recA promoter were grown as in A. At each time point, 0.1 ml of culture was removed and luminescence was quantified using a liquid scintillation counter (⦿ Thymidine, AZT). Values shown are normalized to the A590 of the culture at each time point. (D) As in C, except the plasmid contains the luxCDABE operon under the dinB promoter (⦿ Thymidine, ● AZT).