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. 2011 Feb 28;6(2):e17510. doi: 10.1371/journal.pone.0017510

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Cultures were grown for 24 h and bacterial samples were removed at the indicated time points. Relative ATP levels were measured in bacterial lysates using CellTiter-Glo, a luciferase-based assay kit. (A) Luminescence (Lum) units, proportional to ATP concentration, were calculated for equivolumetric samples. (B) Luminescence units per 10⁶ bacteria at each time point were calculated using the data from panel A, the observed OD₆₀₀ of the culture, and the equation derived from Figure 5D . Each data point is the average of 3 experiments with the standard errors shown. For both graphs, *, p<0.05; **, p<0.01; ***, p<0.001 versus the control condition.