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. 2011 Feb 28;6(2):e17458. doi: 10.1371/journal.pone.0017458

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(a) Section from fourth internode of wild-type Populus stem during primary growth. (b) Section from seventh internode of wild-type Populus stem during transition to secondary growth, showing secondary xylem tissue formation. (c) Section from bottom internode of wild-type Populus stem showing secondary phloem fibers and secondary xylem tissue. (d) Lower magnification view of bottom internode of wild-type stem. (e) Section from fourth internode of 35S::PCN-miRNAd gain of function Populus stem during primary growth, showing increased cambium cell layers. (f) Section from seventh internode of 35S::PCN-miRNAd gain of function Populus stem during transition to secondary growth, showing delayed secondary xylem formation. (g) Section from bottom internode of 35S::PCN-miRNAd gain of function Populus stem showing no lignified phloem fibers formation and decreased xylem tissue. (h) Lower magnification of section from bottom internode of 35S::PCN-miRNAd gain of function Populus stem showing no lignified phloem fibers formation and decreased xylem tissue. (i) Section from fourth internode of 35S::miRNA-PCN knockdown Populus stem showing early formed lignified phloem fibers and xylem cells by comparing with the wild-type. (j) Section from seventh internode of 35S::miRNA-PCN knockdown Populus stem showing increased secondary phloem fibers and xylem tissue formation by comparing with the wild-type. (k) Section from bottom internode of 35S::miRNA-PCN knockdown Populus stem showing ectopic lignifications in pith cells. (l) Lower magnification of section from bottom internode of 35S::miRNA-PCN knockdown Populus stem showing ectopic lignifications in pith cells. Cambial zone (Ca), Phloem (Ph), Phloem fiber (Pf), Xylem (Xy), Bar = 100 µm.