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Transformation and recombination efficiencies with cells grown in either minimal or LB medium:
| Growth medium | Gal+/108 viable1 | Plasmid Transformants/108 Viable2 | Gal+ Recombinants/plasmid transformant |
|---|---|---|---|
| M63 glucose LB | 1.1×104 | 3.0×103 | 4.1% |
| 8.7×106 | 1.4×107 | 34% | |
Values are the average of three or more experiments throughout the table. Recombination was with oligo 144 in HME6.
Transformation was with 20 ng of plasmid pLT60.
