. Author manuscript; available in PMC: 2012 Mar 18.

Published in final edited form as: J Mol Biol. 2011 Jan 19;407(1):45–59. doi: 10.1016/j.jmb.2011.01.030

Table 4.

Effect of recovery/outgrowth time on oligo recombination.

32° outgrowth (min.)	Viability¹	% Recombinants²

0	9.0×10⁵	7.6
15	7.1×10⁶	25.2
30	7.8×10⁶	17.2
45	9.7×10⁶	18.8
60	1.2×10⁷	22.2
75	2.0×10⁷	22.7
90	1.4×10⁷	22.8
120	3.3×10⁷	18.2

Titers determined on L plates at the time shown after electroporation.

Percentage of recombinant colonies on MacConkey galactose plates. Sectored red/white colonies were scored as recombinants. Recombination was with oligo 144 in HME6 cells. Values are an average of three or more experiments throughout the table.