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. Author manuscript; available in PMC: 2012 Mar 18.
Published in final edited form as: J Mol Biol. 2011 Jan 12;407(1):21–34. doi: 10.1016/j.jmb.2011.01.006

Figure 1.

Figure 1

FL (dark bars) and RL constructs (light bar). CGG repeats are located within FMR1 and synthetic 5′UTRs and have the following CGG-repeat lengths (n): 0, 16, 30, 62, 99 CGG. FMR15′UTR(HP)-FL and synthetic 5′UTR(HP)-FL have a strong hairpin (HP) replacing the CGG element. 5′HP-FMR15′UTR(30 CGG)-FL, 5″HP-FMR15′UTR(30 CGG)-FL, and 5′HP-synthetic 5′UTR(30 CGG)-FL each have a strong HP at the 5′ ends of the mRNAs. FL and RL messages are transcribed via SP6 and T7 promoters, respectively.