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. Author manuscript; available in PMC: 2012 Mar 18.
Published in final edited form as: J Mol Biol. 2011 Jan 12;407(1):21–34. doi: 10.1016/j.jmb.2011.01.006

Figure 2.

Figure 2

In vitro translation of capped synthetic and FMR1 5′UTR messages. (A) Synthetic 5′UTR(n CGG or HP)-FL and 5′HP mRNAs. (B) FMR15′UTR(n CGG or HP)-FL, 5′HP and 5″HP mRNAs. All experiments were performed at least in triplicate. Relative FL expression levels were obtained by dividing each FL measurement by the corresponding RL measurement within the same in vitro translation reaction. Using t-tests, all expression levels (within either the FMR1 or synthetic 5′UTR) were significantly different from each other with p<.001, except for the following: FMR1 99 CGG vs. HP p=.414. Synthetic 16 CGG vs. 30 CGG p=.709.