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. Author manuscript; available in PMC: 2012 Mar 18.
Published in final edited form as: J Mol Biol. 2011 Jan 12;407(1):21–34. doi: 10.1016/j.jmb.2011.01.006

Figure 5.

Figure 5

Effect of transcription start site (length of 5′UTR 5′ to the CGG repeat) on translation efficiency of FMR1 message. In vitro translations of FMR15′UTR(n CGG)-FL (Inr I) and FMR1(InrIII)5′UTR(n CGG)-FL mRNAs with either 0, 16, 30, 62, or 99 CGG repeats. mRNAs were transcribed from start site I (dark bars) or start site III (light bars). T-tests of site I vs. site III for each CGG-repeat length were significant, with all P<0.001. Each translation was performed in quadruplicate.