Table 1.
FMR1- and synthetic-5′UTR FL mRNAs used in the current study. All FL mRNAs were generated by SP6-promoted in vitro transcription and are 5′ m7G-capped
| RNA Product | Mid-5′UTR Insert | Additional Modifications |
|---|---|---|
| Inr I; FMR15′UTR(n CGG or HP)-FL | 0, 16, 30, 62, 99 CGGs; HP, HP(GAG) | |
| Inr III; FMR15′UTR-FL(n CGG)-FL | 0, 16, 30, 62, 99 CGGs | |
| Synthetic 5′UTR(n CGG or HP)-FL | 0, 16, 30, 62, 99 CGGs; HP, HP(GUG/no stop) | |
| Inr I; 5′HP-FMR15′UTR(30 CGG)-FL | 30 CGGs | 5′HP, 5″HP |
| 5′HP-Synthetic 5′UTR(30 CGG)-FL | 30 CGGs | 5′HP |
| Synthetic 5′UTR(0 CGG)-FL[-AUG] | 0 CGGs | k.o. AUG start codons |