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. Author manuscript; available in PMC: 2012 Mar 18.
Published in final edited form as: J Mol Biol. 2011 Jan 12;407(1):21–34. doi: 10.1016/j.jmb.2011.01.006

Table 3.

Literature values for hairpin stability and degree of inhibition of translation.38; 39; 40; 76; 77; 78; 79; 80

ΔG (kcal/mol) % Inhibition System Measured by
−11.0 17 Drosophila S2 cells [35S] Hsp70/mRNA 79
−13.4 none NTera2D1 cells chemilum/cell extracts 76
−14.2 74 RRL CAT activity 78
−18.0 83–90 S. cerevisiae CAT or FL activity 77
−22.0 92 Drosophila S2 cells [35S] Hsp70/mRNA 79
−30.0 0–20 Cos cells [35S] proinsulin 40
−30.0 none RRL [35S] CAT 39
−49.4 75 RRL CAT activity 78
−50.0 85–95 Cos cells [35S] proinsulin 40
−50.0 90–95 Xenopus embryos CAT activity 80
−61.0 100 RRL [35S] CAT 39
−61.1 61 RRL, wheat germ TK activity 38
−61.1 82 Cos cells TK activity 38
−74.8 79 NTera2D1 cells chemilum/cell extracts 76
−195.3 93 Cos cells TK activity 38
−195.3 >99 RRL, wheat germ TK activity 38

Abbreviations: CAT, chloramphenicol acetyl transferase; Hsp70, 70 kDa heat shock protein; TK, thymidine kinase; chemilum, chemiluminescence.