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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Int J Parasitol. 2010 Nov 28;41(3-4):355–362. doi: 10.1016/j.ijpara.2010.10.005

Fig. 1.

Fig. 1

Firefly luciferase (luc) activity in homogenates of cultured larvae exposed to calcium phosphate – pBmHSP70(−659 to−1)/luc co-precipitates. Larvae were cultured in the presence of calcium phosphate - pBmHSP70(−659 to−1)/luc co-precipitates for varying periods and homogenates assayed for firefly luciferase activity as described in Materials and methods. Each culture contained 200 L3s. “Background” represents the activity of 200 L3s cultured in the absence of the transfecting DNA. RLU, relative light units. The vertical arrow indicates the day upon which ascorbic acid was added to the cultures to induce molting (Rajan et al., 2003). Columns represent the mean and error bars indicate the S.D. from multiple assays (2–6) run at each time point.