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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Int J Parasitol. 2010 Nov 28;41(3-4):355–362. doi: 10.1016/j.ijpara.2010.10.005

Fig. 2.

Fig. 2

Gaussia princeps luciferase (GLuc) activity in the culture media of embryos biolistically transfected with pBmHSP70(−659 to −1)/GLuc and pBmHSP70/GLuc. Isolated embryos were biolistically transfected with the two constructs and cultured for 48 h. The culture medium was assayed for GLuc activity as described in Materials and methods. The embryos were harvested and their protein content determined using the Bradford method (Bradford, 1976). Activities are expressed as the number of relative light units (RLU) from the medium normalized to the amount of parasite protein in each well. Columns indicate the mean and error bars the S.D.s of six independent transformations.