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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Cell Neurosci. 2011 Jan 15;46(3):671–680. doi: 10.1016/j.mcn.2011.01.008

Fig 5.

Fig 5

Gata3 is suppressed by CNTF and rescues NET transcription. CNTF suppresses Gata3 mRNA in sympathetic neurons (A) and decreases Gata3 protein (B) in M17 cells. Gata3 levels were normalized to β-Actin levels and shown as a percent of control (mean values ± SEM, n=3). (C) A representative western blot showing Gata3 (top panel) and β-Actin (bottom) protein levels. (D) M17 cells were treated for 2 days with vehicle (Control) or CNTF. Cells were then transfected with NET-Luc+pRL-Null to monitor transcription, together with a control vector or Gata3 expression plasmid (Gata3 + CNTF) and maintained for two more days in CNTF or control medium. Data are a ratio of firefly luciferase/renilla luciferase. Data are mean values ± SEM of triplicate values. Each experiment was repeated at least 3 times. *p<0.05, **p<0.01, ***p<0.001.