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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mol Cell Neurosci. 2011 Jan 15;46(3):671–680. doi: 10.1016/j.mcn.2011.01.008

Fig 6.

Fig 6

Hand2 is suppressed by CNTF and rescues NET transcription. CNTF suppresses Hand2 mRNA in sympathetic neurons (A) and decreases Hand2 protein (B) in M17 cells. Hand2 levels were quantified in the same cells analyzed for Gata3, and Hand2 protein was normalized to the β-Actin shown in Fig 5C. Data shown are percent of control (mean values ± SEM, n=3). (C) A representative western blot showing Hand2 (top panel). (D) M17 cells were treated for 2 days with vehicle (Control) or CNTF. Cells were then transfected with NET-Luc+pRL-Null to monitor transcription, together with a control vector or Hand2 expression plasmid (Hand2 + CNTF) and maintained for two more days in CNTF or control medium. Data are a ratio of firefly luciferase/renilla luciferase. Data are mean values ± SEM of triplicate values. Each experiment was repeated at least 3 times. *p<0.05, **p<0.01.