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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Autoimmun. 2011 Jan 22;36(2):115–124. doi: 10.1016/j.jaut.2010.12.003

Figure 4. Neuroantigen-specific suppressive ability is deficient during acute MS exacerbation.

Figure 4

Ex vivo-purified, CFSE-stained CD4+CD25- T-cells from HC, quiescent MS patients or MS patients suffering from an acute exacerbation were used as responders in autologous suppression assays. Panel A shows CFSE vs. CD25 dotplots from representative subjects responding to two neuroantigens (MOG-pool 1 and PLP-pool 1) in the absence of suppressor cells (1:0) or with CD8+ T-cells added at indicated ratios. Red numbers at the top of each dotplot represent proliferative response, whereas the black numbers represent the calculated %suppression. This is representative 15 HC, 11 quiescent MS patients (9 responders) and 9 acute MS exacerbation patients (6 responders), equivalent to 50, 47, and 37 flow-based suppression assays, respectively. Panels B, C and D show cumulative %suppression data at the 1:0.25 responder:suppressor ratio from assays containing neuroantigens, foreign antigens or anti-CD3, as indicated.