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. 2011 Feb 24;5:8. doi: 10.3389/fnana.2011.00008

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Copyright © 2011 Alonso-Nanclares, Kastanauskaite, Rodriguez, Gonzalez-Soriano and DeFelipe.

This is an open-access article subject to an exclusive license agreement between the authors and Frontiers Media SA, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.

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Correlative light and electron microscopy of the subiculum region from a sclerotic hippocampus. (A) Photomicrograph of a 2-μm-thick semithin plastic section stained with 1% toluidine blue to show the numerous pyramidal cells. (B) Low-power electron micrograph taken after sectioning the semithin section shown in A, and illustrating the same pyramidal neuron (py) indicated with an arrow in (A). (C) Higher magnification of the adjacent neuropil shown in (B) to illustrate the numerous synapses. (D) Higher magnification of the asymmetric synapse (indicated with an arrow in C); ds, dendritic spine; t, axon terminal. Scale bar [in (D)]: 120 μm in (A), 58 μm in (B), 1 μm in (C), and 0.5 μm in (D).