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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Neuropsychopharmacology. 2007 Nov 28;33(10):2313–2323. doi: 10.1038/sj.npp.1301635

Figure 2.

Figure 2

Excitatory postsynaptic currents (EPSCs) are depressed by α1-AR agonist application. (a) Whole cell voltage clamp (−70 mV holding potential) experiments were performed to assess long-term depression (LTD) in the dorsal lateral BNST (dlBNST). Methoxamine (100 µM) was applied for 15 min and washed out for 40 min resulting in LTD (N = 6). (b) Under the same conditions 100 µM methoxamine also induced LTD in the ventral lBNST (vlBNST). (N = 8) (c) A lower concentration of methoxamine (10 µM) applied for the same duration also can induce significant LTD in the dlBNST. (d) A representative experiment in the dlBNST: EPSC (pA) = EPSC amplitude, HI (pA) = holding current, RA (MΩ) = Access Resistance, and RI (GΩ) = Input resistance. (d inset) Representative traces of EPSCs, each a 2 min average of baseline (black line) and LTD (gray line; scale bar 40 pA by 5 ms). (e) Applying 100 µM methoxamine after previous induction of LTD fails to further depress EPSCs (N = 6).