Skip to main content
. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Biol Chem. 2007 Sep 20;282(46):33769–33775. doi: 10.1074/jbc.M700807200

FIGURE 7. The Fis1 arm regulates access to a Dnm1 binding pocket.

FIGURE 7

A, GST pull-down experiments between point mutants of ΔN16Fis1 and MBP-Dnm1. The binding is reported as fraction of Dnm1 bound, which is measured as the ratio of anti-MBP to anti-GST relative to ΔN16Fis1-GST binding of MBP-Dnm1 (lane 2). The error bars represent the S.D. from at least three independent experiments. Experiments were performed by GST pull-down as in Fig. 2A. B, structural representation of Dnm1 binding data showing Fis1 mutants that did (red) or did not (blue) affect Dnm1 binding. Residues that were not tested are colored light gray. The Fis1 arm was not present in these experiments but is shown in yellow for illustrative purposes. The figure was made using PyMOL (W. L. DeLano; available on the World Wide Web).