Figure 1.
Rapid changes in IL-17A and IL-6 serum cytokine concentrations in C57BL/6J mice following vector cannulations. Sera were prepared from blood collected from individual five-week old mice (n = 4) randomly chosen one week prior to vector treatment (Day 0 on the graph). Mice were allowed to acclimate for seven days, followed by vector instillation of each salivary gland with 50 μl of vector solution containing 107 viral particles of either Ad5-LacZ or Ad5-IL17A vector. Sera were again prepared from blood collected from individual mice (n = 11) at Day 5 and Day 12 post-treatment. Concentrations of cytokines were determined using the Luminex platform. To ensure sufficient quantities for testing, the sera of three individual mice of each experimental group were pooled. ND, not detected indicates levels below threshold detection.