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. 2010 Dec 31;12(6):R230. doi: 10.1186/ar3217

Table 2.

Technical performance of the aggrecan capture OA-1 ARGS ELCL assay

Range of detection of standards 0.075 to 5 pmol ARGS/ml
Minimum required dilution of SF 1:4
Lower limit of detection in SF 0.3 pmol ARGS/ml SF
Parallelism (obtained/expected) of four SF samples diluted 1:4 to 1:4,000a 100% (78% to 120%)b
Spiking recovery of equimolar spiking of seven SF samples 116% (92% to 133%)b
Intra-assay CV, n = 14 3.5%
Inter-assay CV, n = 9 16.6%

CV, coefficient of variation; ELCL, electrochemiluminescence; SF, synovial fluid. aSFs with 9, 95, 131, and 444 pmol ARGS/ml diluted and read within the range of detection of the standard curve. bMean (range).