Table 2.
Technical performance of the aggrecan capture OA-1 ARGS ELCL assay
Range of detection of standards | 0.075 to 5 pmol ARGS/ml |
Minimum required dilution of SF | 1:4 |
Lower limit of detection in SF | 0.3 pmol ARGS/ml SF |
Parallelism (obtained/expected) of four SF samples diluted 1:4 to 1:4,000a | 100% (78% to 120%)b |
Spiking recovery of equimolar spiking of seven SF samples | 116% (92% to 133%)b |
Intra-assay CV, n = 14 | 3.5% |
Inter-assay CV, n = 9 | 16.6% |
CV, coefficient of variation; ELCL, electrochemiluminescence; SF, synovial fluid. aSFs with 9, 95, 131, and 444 pmol ARGS/ml diluted and read within the range of detection of the standard curve. bMean (range).