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. 2011 Jan 4;155(3):1060–1067. doi: 10.1104/pp.110.166793

Table I. Kinetic parameters for HCT2.

Varying Substrate	Saturating Substrate^a	Kinetic Parameter
Varying Substrate	Saturating Substrate^a	K_m	V_max	V_max/K_m
		mm	nkat mg⁻¹	nkat mg⁻¹ mm⁻¹
p-Coumaroyl-CoA	Malate	1.81 ± 0.19	82 ± 8	45
Caffeoyl-CoA	Malate	1.26 ± 0.12	10 ± 1	7.9
Feruloyl-CoA	Malate	1.17 ± 0.14	62 ± 6	53
Malate	p-Coumaroyl-CoA	0.64 ± 0.06	56 ± 7	87
Malate	Caffeoyl-CoA	0.67 ± 0.06	7.2 ± 1.0	11
p-Coumaroyl-malate	CoA	0.20 ± 0.04	0.6 ± 0.1	3
Phaselic acid^b	CoA	nd	nd	nd

^a

Substrates were present at the following concentrations: malate, 3 mm; p-coumaroyl-CoA, 4 mm; caffeoyl-CoA, 4 mm; CoA, 3 mm.

^b

nd, Kinetic parameters not determined because no reaction was detected.