Figure 10.

Changes in the metabolic network (primary and secondary metabolites) of transgenic BnSCE3 seeds. Sinapate leading to sinapine and sinapate derived from BnSCE3 activity in the transgenic seeds might be present within the same metabolite pool. Red compound names indicate significantly increased amounts, blue names indicate reduced amounts, black names indicate unchanged amounts, and gray names indicate amounts not determined. Boxed names point to compounds with largest quantitative changes in both transgenic lines (OE1 and OE2) compared with the wild-type plants, with threshold levels for primary metabolites (Table III) of change fold factors 1.5 and 0.7 and for secondary metabolites (Tables I and II) of change fold factors 20 (4.322; log₂) and 0.1 (−3.322; log₂). For increased amounts of phospholipids, see Figure 8. Arrows indicate both one-step and multistep reactions, based on conceivable and well-known biochemical reactions. The reaction steps of the general phenylpropanoid metabolism are defined according to Humphreys and Chapple (2002), Boerjan et al. (2003), and Weng et al. (2010). G, Guaiacyl moiety; Gent, gentiobiose; Hex, unknown hexose moieties (commonly Glc); Pent, pentose; S, syringyl moiety; Soph, sophorose; [1], Böttcher et al. (2009); [2], Baumert et al. (2005); [3], Wolfram et al. (2010).