Figure 2.

Leaf-closing activities of different jasmonate derivatives using pinnae of S. saman. The leaf-closing assay was carried out using pinna of S. saman containing secondary and tertiary pulvini, which had been isolated from seedlings conditioned to a 16/8-h light-dark cycle for a week. A closing-opening movement of a pair of pinnule continues after they are disconnected from the stem and their behavior was monitored after addition of different jasmonate at 4 h of the second light cycle. The sections shows the status of pinnae at 10 h of the second light cycle after treatment for a period of 6 h with the following compounds: A, Water; B, (−)-LCF (H⁺-form); C, (−)-LCF (K⁺-form); D, (+)-ent-LCF; E, (−)-12-OH-JA; F, (−)-JA; G, (−)-JA-Ile (each at 500 μm); or H, (+)-coronatine (at 5 μm). The experiment was repeated three times with three replicates each.