Figure 6.

Activation of the LOX2 promoter by different jasmonates. Arabidopsis plants homozygous for a single insertion of the reporter gene, comprising a 1.8-kb promoter of the LOX2 gene (Jensen et al., 2002), were grown in liquid culture for 2 weeks and subsequently treated with the indicated compounds (at 20 or 100 μm) for 24 h. Control treatment was with DMSO only. GUS activity was quantified in plant extracts using 4-methylumbelliferyl-β-d-glucuronide as substrate in a fluorimetric assay and related to the protein concentration. Activities are normalized to the control treatment (DMSO) and thus represent fold activation. The data represent the average of at least three biological replicates (±sd) and GUS activities that were significantly different compared to control treatment at P < 0.01 (Student’s t test) are indicated (*). Similar values were obtained using an Arabidopsis line harboring the jasmonate-responsive OPCL1p::GUS reporter gene (Kienow et al., 2008).