Figure 3.

A, Schematic diagram of the genome organization of AtDFB. drh2 had a T-DNA insertion in the fifth intron of the At5g05980 gene, which encodes AtDFB. Boxes indicate exons and lines indicate introns. The T-DNA insertion sites for each of the mutant alleles are indicated. B, Twelve-day-old seedlings of the wild type, atdfb-1, atdfb-2, and atdfb-3. Note the severely reduced primary root length of all mutant alleles. C, Semiquatitative RT-PCR of AtDFB in the wild type (WT), atdfb-1, atdfb-2, and atdfb-3. Total RNA was prepared from 7-d-old seedlings, and arrows in A indicate the positions of the primers used for RT-PCR. EIF4A-2 primers were used as a control.