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. 2011 Jan 13;155(3):1237–1251. doi: 10.1104/pp.110.168278

Figure 7.

Figure 7.

DR5:GFPm expression and QC identity are altered in atdfb roots. A, Root cap of an 8-d-old wild-type seedling shows DR5:GFPm expression in the QC and central columella cells (CC). B, In 8-d-old atdfb-1 seedlings, DR5:GFPm expression is confined to a few columella cells (arrows). Note the disorganized QC region in atdfb-1 (asterisks). C, More cells in the root cap express DR5:GFPm in wild-type seedlings (WT) compared with atdfb-1.Values are means from 15 roots ± se. The asterisk indicates a statistically significant difference (Student’s t test, P < 0.01). D, Purple-stained amyloplasts are absent in columella initials (CI) of wild-type seedlings. E, In roots of atdfb-1, amyloplasts (arrows) prematurely form close to the disorganized cells in the QC region (asterisks). F, Quantitative RT-PCR shows higher expression of the QC-expressed gene AGL42 in 8-d-old roots of the wild type compared to atdfb-1. Values are means ± se of five biological replicates. The asterisk indicates a statistically significant difference according to Student’s t test (P < 0.05). Bars = 10 μm.