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. 2011 Jan 20;155(3):1252–1263. doi: 10.1104/pp.110.171322

Figure 6.

Figure 6.

Phenotypes of wild-type and transformed seedlings expressing 35S:IAA12mImII transgenes with a single, double, or triple mutation in domain I. A, Photographs of 7-d-old seedlings. Two representative seedlings are shown for the wild type (Wt) and 35S:IAA12mImII transformants. a and b show two 35S:IAA12mImII-1 primary transformants with fused cotyledons and stunted primary root, respectively. The wild type, 35S:IAA12mImII-2, and 35S:IAA12mImII-3 are at the same magnification. a and b for 35S:IAA12mImII-1 are at the same magnification. Bars = 0.5 cm. B, Histochemical GUS staining of 7-d-old wild-type and transformed seedlings. Seedlings were stained for 15 h at 37°C. a and b show two 35S:IAA12mImII-1 primary transformants with weaker GUS staining. C, Hypocotyl length for 7-d-old wild-type and transformed seedlings. Error bars indicate sd (n ≥ 15, ANOVA test, P < 0.05). Lanes are as follows: a, the wild type; b, 35S:IAA12mImII-2; c, 35S:IAA12mImII-3. D, Length of primary roots for 7-d-old wild-type and transformed seedlings. Error bars indicate sd (n ≥ 15, ANOVA test, P < 0.05). Lanes are as follows: a, the wild type; b, 35S:IAA12mImII-2; c, 35S:IAA12mImII-3. E, Photograph of a wild-type plant (left) and a T1 35S:IAA12mImII-1 transformant (arrow). Bar = 1 cm. The inset at right shows an enlargement of the T1 transformant.