ZmPep1 activates defense gene expression. A, Average fold changes in transcript abundance over time in excised leaves treated with water or ZmPep1 (2 nmol g−1 fresh weight) versus untreated leaves. B, Dose dependence of ZmPep1-induced transcriptional changes in excised leaves at 4 h with actin amplification as a control. FW, Fresh weight. C, Local average changes in defense gene transcription in intact plants 4 h after application of water, 25 pmol of ZmPep1, or pectinase elicitor to a wound site. D, Local average changes in transcript abundance in intact plants 24 h after inoculation with 5 × 103 C. heterostrophus spores. For graphs in A, at the time point of greatest mean change, different letters (a and b) represent significant differences (all ANOVAs, P < 0.02; Tukey test corrections for multiple comparisons, P < 0.05). For graphs in C and D, asterisks represent significant differences from the water-treated control (P < 0.05). n.s.d. (not statistically different) indicates ANOVA P > 0.05. For all graphs, n = 3 (±se).