Figure 5.

ZmPep1-induced defense-associated metabolites. A, Average anthranilate and indole accumulation in excised leaves. B, Dose dependence of ZmPep1-induced anthranilate and indole accumulation in excised leaves. C, Time course and dose dependence of ZmPep1-induced changes in expression of the ASsub2 gene. Transcript abundance was normalized via comparison with an actin control and expressed as fold change relative to an untreated leaf. For all experiments, unless otherwise indicated, ZmPep1 was supplied at 2 nmol g⁻¹ fresh weight. Each sample was a pool of two leaves. For graphs in A and C, at the time point of greatest mean change, different letters (a and b) represent significant differences (all ANOVAs, P < 0.02; Tukey test corrections for multiple comparisons, P < 0.05). For graphs in B, asterisks represent significant differences from the water-treated control (P < 0.04). n.s.d. (not statistically different) indicates ANOVA P > 0.05. For all graphs, n = 3 (±se). FW, Fresh weight.