Figure 2.

Increased expression of AHb2 leads to an increase in polyunsaturated fatty acids in Arabidopsis seeds. Mature seeds of wild-type plants (Wt; black bars) and transgenic Arabidopsis plants overexpressing AHb2 in the T3 generation (gray bars) were analyzed to determine total fatty acids in μg seed⁻¹ (A), C20:1 eicosenoic acid (a marker for TAG) in μg seed⁻¹ (B), C18:1 oleic acid in μg seed⁻¹ (C), C18:2 linoleic acid in μg seed⁻¹ (D), C18:3 linolenic acid in μg seed⁻¹ (E), C18:2/C18:1 ratio (F), C18:3/C18:2 ratio (G), C18:3 in mol % of total fatty acids (H), and USI of seed lipids (I) according to Gutierrez et al. (2005). Values are means ± se (n = 10 different sets of plants). Changes that are significantly different from the wild type (based on Student’s t test with P < 0.05) are labeled with asterisks. The weights of mature seeds (in μg seed⁻¹) were as follows: 15.1 ± 0.89, 16.54 ± 0.79, 13.32 ± 1.0, and 16.85 ± 1.6 for the wild type, line 9, line 10, and line 11, respectively.