FIG. 3.

AICAR stimulates glucose uptake and storage into glycogen. EDL muscles from C57BL/6J mice were incubated with vehicle, 2 mmol/L AICAR, or 10 mU/mL insulin for 40 min in KRB containing 2 mmol/L pyruvate. A: Muscles were transferred to vials containing 2-deoxy-[³H]glucose and glucose transport assayed as described in research design and methods. B: Alternatively, EDL muscles incubated with vehicle or 2 mmol/L AICAR for 40 min were snap frozen in liquid nitrogen and glucose-6-phosphate (G6P) levels assayed as described in research design and methods. C: Isolated EDL muscles were incubated with the indicated stimuli for 40 min in KRB containing d-[U-¹⁴C]glucose (5.5 mmol/L), and the rate of glucose incorporation into glycogen was determined as described in research design and methods. D: Alternatively, EDL muscles were incubated in KRB containing unlabeled glucose (5.5 mmol/L) for 1 h, and glycogen content was determined by digestion of KOH extracts with amyloglucosidase as described in research design and methods. E: EDL muscles were incubated with vehicle or 2 mmol/L AICAR for 40 min and lysates immunoblotted with the indicated antibodies. Results are presented as mean ± SEM from the indicated number (n) of mice. *P < 0.05 compared with basal.